Quantification of Motor-neuron Adaptation to Sustained and Intermittent Stimulation

In deeply anesthetized mammals, as typified by the adult cat, there is limited evidence that the firing-rate response of spinal motor neurons to sustained stimulation usually features a progressive reduction in firing rate, termed late adaptation, that begins 1 2 s after the onset of sustained stimulation. The fullest description of late adaptation has been provided by Kernell & Monster (1982a,b) who evoked repetitive firing in spinal motor neurons of deeply anesthetized cats by the conventional procedure of an intracellular injection of a sustained depolarizing current. The main purpose of the present study was to extend on the results of their work. The first hypothesis tested was: Sustained depolarizing extracellular stimulation of motor neurons is more effective in maintaining repetitive discharge than sustained depolarizing intracellular stimulation. Investigations pioneered by Kernell & Monster (1982a,b) tested the association between late adaptation and other type (size)-related properties of motor neurons. Such analyses are within the rubric of Henneman's (1957, 1977) Size Principle, one component of which proposes that the properties of motor neurons and the muscle fibers they innervate are tightly coupled. The second hypotheSiS was proposed to continue this inquiry. It stated that: Late adaptation (during both sustained and intermittent stimulation), and other discharge-related properties of motor neurons are associated with other type (size)-related properties of these cells and their motor units. For both hypotheses, there was an emphasis on providing a quantitative description of late-adaptation. In the present study, the duration of repetitive firing in response to sustained stimulation significantly exceeded that in the Kernell & Monster (1982a,b) study. thereby providing evidence In support of the first hypothesis. For sustained stimulation, significant associations were found between the time constant of late adaptation and three neuromechanical properties of the cell's motor unit: axonal conduction velocity; twitch contraction time; and, peak tetanic force. Similarly,